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The scRNA/CITE-seq libraries were prepared using the GemCode Single Cell 3 Gel Bead and Library kit, version 3 (10x Genomics 1000128) according to the manufacturers instructions with the addition of amplification primer (3 nM, 5CCTTGGCACCCGAGAATT*C*C) during cDNA amplification to enrich the TotalSeq-A cell surface protein oligos

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doi: 10.1016/j.envint.2022.107357

After 6 h of illumination, there was a significant difference in cell survival rate between the model group and the control group, indicating successful modeling ( 3.1.2 ARPE-19 cell model The ARPE-19 cell line is derived from adult retinal pigment epithelial (RPE) explants, and ARPE-19 cells can be extensively expanded, providing a relatively stable source of retinal epithelial cells for functional and genetic research ( 2 for 4 h, the barrier function was impaired (Table 1)

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