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Siegel, R., Ma, J., Zou, Z

doi: 10.1016/j.bbi.2015.05.011 199 SPHoffmannARotheTOuyangZBaumWStaszewskiOet al

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[64] At least one study suggests that orally administered GABA increases the amount of human growth hormone (HGH)

The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000, Proteintech, SA00001-2, Chicago, USA) at room temperature for 1 h, and the signals were visualised using an enhanced chemiluminescence detection reagent (Sigma-Aldrich, USA)