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T reg differentiation For regulatory Tcell (T reg ) differentiation, CD4 + T cells were incubated for 5 days using TexMACS TM medium (# 130097196, Miltenyi Biotec, Bergisch Gladbach, Germany), stimulated with T Cell TransAct TM human (Miltenyi Biotec, Bergisch Gladbach, Germany) and supplemented with 5 ng/ml TGF1, 1 g/ml antiIFN, 1 g/ml antiIL4, 100 U/ml IL2 (#130 095067 | #130 095743 | # 130095709 | #130 097 742, Miltenyi Biotec, Bergisch Gladbach, Germany), and 10 mM of retinoic acid ( #R2625

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The peptide modulates dopamine D2 receptors and serotonin pathways, both of which regulate sleep architecture (Sikiri et al., 1999)