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Quantification of reduced GSH using DTNB revealed that intracellular GSH levels markedly decreased following RPTS exposure, with a reduction of 61.0% in Saos-2 cells (Control: 19.67 2.52 to RPTS: 7.67 1.53) and 66.1% in MG-63 cells (Control: 20.67 2.08 to RPTS-H: 7.00 1.00) ( Figure 2C )