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However, glutamic acid did not contribute to pH transition, likely because its negatively charged side chain in the mobile phase resists donating H + to the stationary phase, resulting in a lower-than-expected pH shift in the final elution fraction

Athletes are required to report to doping control immediately, unless a valid reason is discussed with and permitted by a DCO

All mixtures were subjected to SDS-PAGE electrophoresis for GSDME activation assay

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