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The dsDNA handle was prepared by PCR (see Supplementary Table 3 for oligonucleotides) including 200 M final concentration of each dNTP (dGTP, dCTP, dATP), 140 M dTTP and 66 M Bio-16-dUTP (Roche) using the plasmid pSP73-JY0 as template 80 followed by digestion with the restriction enzyme BsaI

We found that the amount of AngIV in mouse tissue increased after the administration of Dihexa compared to that in the WT group

But, it doesnt necessarily mean that increased B12 levels cause weight loss