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Description

coli BL21(DE3) using 0.5 mM IPTG induction at 37C for 6 h and purified initially using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva)

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Once reconstituted with bacteriostatic water, refrigerate and use within 2830 days

Clin Toxicol 2012

For instance, bile salts are surfactants with potent reducing power and therefore would interfere with the oil/water interface and ROS reactions

Knockdown of AATF led to an accumulation of -H2AX at 12 h post-AATF-KD, while an increase in cleaved caspase-3 levels was observed from 36 h post-AATF-KD (Supplementary Fig