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1 Remove BPC-157 (Oral) vial from refrigeration and allow to reach room temperature (1520 minutes) 2 Clean vial top with alcohol swab and allow to air dry completely 3 Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water) 4 Inject BAC water slowly down the side of the vial do not aim directly at the powder 5 Gently swirl in circular motions DO NOT shake vigorously as this degrades the peptide 6 Allow 23 minutes for full dissolution solution should be clear and colorless 7 Draw calculated dose into an insulin syringe for subcutaneous injection 8 Inject into abdomen, thigh, or upper arm

Due to inconsistent units of measurement across studies, the standardized mean difference (SMD) was adopted as the common effect size metric, with Hedges g used for small-sample correction (Lifeng Lin and Aloe, 2021)

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Water has a pH of 7, so this is the point of neutrality on the pH scale

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